Successful results of both procedures raised the total number of NWR embryos produced to 22-all of them from female Fatu, with half of them sired by the deceased male Suni who was born in Safari Park Dvůr Králové, Czech Republic, and the other half sired by Angalifu who lived in San Diego Zoo Safari Park, U.S. Out of 16 collected oocytes, three embryos were produced in the Avantea laboratory, again using the semen of Angalifu. This collection followed on from the 9th oocyte collection held at the same location and by the same team on April 24th. Eventually, five embryos of Fatu were successfully produced and cryopreserved in liquid nitrogen. Following maturation, seven of the oocytes were fertilized using cryopreserved, thawed semen from the deceased NWR male Angalifu. The oocytes were immediately air-lifted to the Avantea laboratory in Cremona, Italy. Oocyte collections from Najin, Fatu's mother, were ceased in 2021 following an in-depth ethical risk assessment. The BioRescue team was able to collect 23 oocytes from Fatu, the younger of the two remaining NWR females. The 10th oocyte collection in northern white rhinos (NWR) was performed by a team of scientists and conservationists from the Leibniz Institute for Zoo and Wildlife Research (Leibniz-IZW), Ol Pejeta Conservancy, Safari Park Dvůr Králové, Kenya Wildlife Service (KWS) & Wildlife Research and Training Institute (WRTI) on July 28, 2022, at Ol Pejeta Conservancy in Kenya. Regular veterinary and ethical assessments of oocyte collection procedures show that Fatu handles the procedures well and shows no signs of detrimental health effects. At the same time, the consortium places the highest value on respecting the life and welfare of the individual animals involved. This nourishes the hope to eventually succeed in producing new offspring and give a keystone grazer of Central Africa a new future.
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